Life is a thing taken for granted by most. However, it is the life-long quest of many to unravel the mysteries of it. Understanding and characterizing the incomprehensively complex molecular interaction networks within a biological organism, which defines that organism, is a vital prerequisite to understand life itself…
Contents
1 GENERAL INTRODUCTION
2 BIOMOLECULES AND BIOMOLECULAR RECOGNITION
2.1 DNA
2.2 PROTEINS
2.2.1 Antibodies
2.2.2 Receptors
2.3 LIPIDS
3 BIOSENSORS – MONITORING BIOMOLECULAR INTERACTIONS
3.1 SPONTANEOUS PROTEIN ADSORPTION TO SOLID SURFACES
3.2 CONTROLLED PROTEIN IMMOBILIZATION
3.2.1 Covalent Immobilization
3.2.2 Immobilization of Biotinylated Proteins
3.2.3 Protein Immobilization via Antibodies
3.2.4 Immobilization of Histidine-tagged Proteins
3.3 MONITORING BIOMOLECULAR INTERACTIONS IN REAL TIME
3.4 TOWARDS HIGHER THROUGHPUT WITH MICROARRAY CHIPS
3.4.1 Functional Proteomics
3.4.2 Pharmaceutical Research and Drug Discovery
3.4.3 Clinical Diagnostics
3.4.4 Trace Detection of Harmful or Illegal Substances
4 SURFACE MODIFICATIONS
4.1 SELF-ASSEMBLED MONOLAYERS (SAMS) OF THIOLS
4.2 PATTERNING
4.2.1 Microcontact Printing
4.2.2 Ink-jet Printing
5 INSTRUMENTATION
5.1 INFRARED REFLECTION ABSORPTION SPECTROSCOPY (IRAS)
5.2 ELLIPSOMETRY
5.3 IMAGING ELLIPSOMETRY
5.4 SURFACE PLASMON RESONANCE (SPR)
5.5 IMAGING SPR
6 INCLUDED PAPERS
7 REFERENCES
Author: Klenkar, Goran
Source: Linköping University
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