The kidney is the key body organ controlling the extracellular fluid volume and thus the arterial blood pressure. The neuronal isoform of nitric oxide synthase (nNOS) in the kidney is primarily located in the macula densa cells. These cells are sensors both for renin release and the tubuloglomerular feedback mechanism (TGF), that is an essential regulator of the glomerular filtration rate and afferent arteriole tone. The purpose of this investigation ended up being to elucidate the purpose of nNOS in the macula densa cells. Acute nNOS inhibition in rats led to an increased TGF responsiveness and unchanged blood pressure while, after chronic inhibition, the TGF was normalised and also the blood pressure was elevated. The plasma renin concentration was raised in rats on long-term low salt diet, but wasn’t considerably impacted by chronic nNOS inhibition. On the contrary, nNOS inhibition for 4 days increased plasma renin concentration in rats treated with a low salt diet. The renal vasculature of rats demonstrates a reduced renal blood flow and intracellular Ca2+ response to angiotensin II after one week blockade of nNOS while angiotensin II’s impact on the renal the flow of blood was eliminated after a month treatment…
Contents: Macula Densa Derived Nitric Oxide and Kidney Function
INTRODUCTION
AUTOREGULATION
Myogenic response
Tubuloglomerular feedback
NITRIC OXIDE
RENIN-ANGIOTENSIN SYSTEM
AIMS OF THE INVESTIGATIONS
MATERIALS AND METHODS
ANIMALS
SURGICAL PREPARATION (STUDIES I, II, III & IV)
TAIL-CUFF BLOOD PRESSURE MEASUREMENTS (STUDIES I & II)
WHOLE KIDNEY CLEARANCE MEASUREMENTS (STUDIES I, II & IV)
STOP-FLOW PRESSURE MEASUREMENTS (STUDIES I & IV)
MEASUREMENTS OF SNGFR (STUDIES I & IV)
ISOLATED BLOOD-PERFUSED JUXTAMEDULLARY NEPHRON PREPARATION (STUDY I)
SAMPLING AND RENIN ASSAY (STUDY II)
RENAL BLOOD FLOW MEASUREMENTS (STUDY III)
MEASUREMENTS OF CYTOSOLIC CALCIUM CONCENTRATION (STUDY III)
DISSOLVING 7-NI (STUDIES I, II, III & IV)
STATISTICAL ANALYSES (STUDIES I, II, III & IV)
EXPERIMENTAL PROTOCOL….
Source: Uppsala University Library
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