The central theme of this thesis is the use of imaging Surface Plasmon Resonance (iSPR) as a tool in the characterization of surfaces with laterally varying properties. Within the scope of this work, an instrument for iSPR analysis was designed and built. SPR is a very sensitive technique for monitoring changes in optical properties in the immediate vicinity of a sensor surface, which is very useful in biosensing and surface science research. We have employed SPR in the Kretschmann configuration, wherein surface plasmons are excited by means of an evanescent field arising from total internal reflection from the backside of the sensor surface. In iSPR, the signal is the reflectivity of TM-polarized light which is measured using an imaging detector, typically a CCD camera. Advantages of this technique include extreme surface sensitivity and, because detection is done from the backside, compatibility with complex samples. In addition, SPR is a non-labeling technique, and in imaging mode….
Contents
1 General introduction
1.1 Imaging methods in biosensing
1.2 Biomolecules and biomolecular interactions
1.3 On the significance of gradients
2 Surface plasmon resonance
2.1 Theory
2.2 Stratified medium matrix model
2.3 Optical SP excitation
2.4 Imaging surface plasmon resonance
2.5 Imaging SPR and electrochemistry
3 Soft matter surfaces
3.1 Self assembled monolayers
3.2 Matrix surfaces
3.3 Surface pattern techniques
4 Instrument design
4.1 Optical setup
4.2 Fluidics and cell for electrochemistry
4.3 Interface
4.4 Data processing and analysis
4.5 Performance
5 Future perspectives
Bibliography
Author: Andersson, Olof
Source: Linköping University
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