The CNS monoaminergic systems are considered as the head engine regulating neuropsychiatric functions and personality. Transcription factor AP-2 is known to be essential for the development of the brainstem including the monoaminergic nuclei, and has the ability to regulate many genes in the monoaminergic systems. The ability of transcription factors to regulate specific gene expression, has lately made them hot candidates as drug targets. In this thesis, results indicating a role of AP-2 in the molecular effects…
Contents
1. INTRODUCTION
1.1. The CNS serotonergic system
1.1.1. The serotonergic cell nuclei and their projections
1.1.2. The serotonin synapse- serotonin metabolism and the 5-HT receptors
1.1.3. The serotonergic system and behavior
1.2. The CNS noradrenergic and dopaminergic systems
1.3. Transcription factors
1.3.1. What is a transcription factor?
1.3.2. Transcription factors as potential drug targets
1.4. Transcription factor family AP-2
1.4.1. Structure and function of transcription factor AP-2
1.4.2. AP-2 and brain embryonic development
1.4.3. AP-2 in the adult brain
1.4.4. Polymorphisms in the AP-2ȕ gene
1.5. Depression and antidepressants
1.5.1. Theories of depression
1.5.2. Antidepressant drugs
1.5.3. Animal models of depression
1.6. Platelet monoamine oxidase (platelet-MAO)
2. PRESENT INVESTIGATION
2.1. Aims of the thesis
2.2. Methodological considerations
2.2.1. Animals and treatment paradigms
2.2.2. Preparation of nuclear extracts
2.2.3. Electrophoretic mobility shift assay (EMSA)
2.2.4. Enzyme-Linked Immunosorbent assay (ELISA)….
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Source: Uppsala University Library