The important protein hormone insulin, responsible for different kind of functions in our body but mainly storage of nutrients, has for a long time been used for treatment of diabetic patients. This important protein is both physically and chemically unstable. Especially during production where the insulin protein is exposed to unnatural environmental conditions such as acidic pH has this been causing problems since huge volumes of the product go to waste.In the human body…
Contents
1. Insulin molecule and amyloid fibril formation
2. How and why the kinetic experiment was going to be done. Expected effects of seeds and surfaces
3. What instruments were being used to study kinetics of insulin amyloid fibrillation
4. Experimental procedures
4.1. Experiment 1
4.1.1. Making the stock solution
4.1.2. Making the surfaces
4.1.3. Kinetic experiment
4.1.4. Fluorescence measurements
4.2. Experiment
4.2.1 Making the stock solution
4.2.2. Making the surfaces
4.2.3. Kinetic experiment
4.2.4. Fluorescence measurements
5. Difference between washed and not washed stained surface
6. Discussion and source of error – what could have been done better?
7. References
Appendix I
Appendix II
Appendix III
Appendix IV
Appendix V
Appendix VI
Appendix VII
Appendix VIII
Author: Salagic, Belma
Source: Linköping University
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